Hydroxyproline as a Marker of the Efficiency of Skin Wound Therapy in Ischemic Conditions
The aim of the study was to analyze the level of hydroxysoplin in tissues as a marker of healing efficiency of experimental skin wound under ischemia conditions. A wound was reproduced according to our developed method on 90 rats Vistar. There were 3 groups of rats of 30 individuals. Therapy was not carried out in the group «Control», local treatment with multi-component means was performed in the «First experimental» group, magnetotherapy was performed with the above-mentioned agent in the «Second experimental» group. The hydroxylol content was estimated based on Newman’s, Logan’s technique modified by Cissel. The results were compared with planimetric, visual and morphological data. The formation of scar was observed in each series on the 10th day, there was recorded the predominance of mature connective tissue over granulation in the experimental groups in contrast to the «Control» group. There was a strong correlation between the hydroxyprolin content and the wound area in all groups. The article marked the reliability of the method of quantitative analysis of hydroxyproline, which made it possible to increase the objectivity of the representation of the course of the wound process in conjunction with other methods of research.
Lipatov V.A., Naimzada M.D.Z., Terehov A.G., Zajtsev A.I., Litvinenko V.Yu., Mishina E.S., Grigoryan A.Yu. 2024. Hydroxyproline as a Marker of the Efficiency of Skin Wound Therapy in Ischemic Conditions. Challenges in Modern Medicine, 47(2): 273–288 (in Russian). DOI: 10.52575/2687-0940-2024-47-2-273-288
While nobody left any comments to this publication.
You can be first.
Bezhin A.I., Lipatov V.A., Fronchek Je.V., Grigorjan A.U., Naimzada M.D.Z., Lazarenko E.D., Medvedeva M.A. 2019. Vliyanie hitozan-kollagenovogo kompleksa s nanochasticami serebra na techenie ranevogo processa v jeksperimente [The Influence of Chitosan-Collagen Complex with Silver Nanoparticles on the Course of the Wound Process in an Experiment]. Chelovek i ego zdorov`e, (2): 5–16. doi: 10.21626/vestnik/2019-2/01.
Grigorjan A.U., Bezhin AI., Pankrusheva T.A., Zhilajeva L.V., Mishina E.S., Chekmareva M.S. 2023. Jeksperimental`naya ocenka osobennostei techenija ranevogo processa pri ispol'zovanii original`nogo ranevogo pokrytij [Experimental Assessment of the Features of the Course of the Wound Process when Using an Original Wound Covering]. Sibirian Medical Review, 141: 93–99. doi: 10.20333/25000136-2023-3-93-99.
Ivanov I.S., Temirbulatov V.I., Kletov M.E., Okunev O.A., Ivanova I.A. 2018. Sootnoshenie kollagena I i III tipov v legkih i kozhe u pacientov s bulleznoj emfizemoj legkih, oslozhnennoj spontannym pnevmotoraksom [The Ratio of Collagen Types I and III in the Lungs and Skin in Patients with Pulmonary Bullous Emphysema Complicated by spontaneous pneumothorax]. Surgery News, 3: 293–300. doi: 10.18484/2305-0047.2018.3.293.
Maksimov A.V., Kir'janova V.V. 2019. Magnitnaja terapija v klinicheskoj praktike [Magnetic Therapy in Clinical Practice. Physiotherapy, Balneology and Rehabilitation]. Fizioterapija, bal'neologija i reabilitacija, 18(6): 412–426. doi: 10.17816/1681-3456-2019-18-6-412-426.
Morgun E.I., Rogovaja O.S., Voroteljak E.A. 2018. Model' ishemizirovannoj dlitel'no nezazhivajushhej kozhnoj rany: kletochnaja gibel' i mehanizmy ranozazhivlenija. Sovremennye tehnologii v medicine [Model of Ischemic Long-Term Non-Healing Skin Wound: Cell Death and Wound Healing Mechanisms]. 10(4): 69–77. doi: 10.17691/stm2018.10.4.08.
Muromceva E.V., Sergackij K.I., Nikol'skij V.I., Shabrov A.V., Al'dzhabr M., Zaharov A.D. 2022. Lechenie ran v zavisimosti ot fazy ranevogo processa [Treatment of Wounds Depending on the Phase of the Wound Process]. Izvestija vuzov. Povolzhskij region. Medicinskie nauki, 3(63): 93–109. doi: 10.21685/2072-3032-2022-3-9.
Put'atina А.N., G.S. Russkih, L.B. Kim L.B. 2020. Sposob opredelenija frakcij gidroksiprolina v biologicheskom material [Method for Determining Hydroxyproline Fractions in Biological Material]. Patent RF № 2735375 C1, МПК G01N 33/52:31.
Nohsorova M.A., Borisova N.V., Ammosova A.M. 2019. Vozmozhnost' diagnostiki nedifferencirovannoĭ displazii soedinitel'noĭ tkani s pomoshh'ju biologicheskih markerov [Possibility of Diagnosing Undifferentiated Connective Tissue Dysplasia Using Biological Markers]. Vestnik novyh medicinskih tehnologij. Jelektronnoe izdanie, 13(4): 138–143. doi: 10.24411/2075-4094-2019-16435.
Sidorenko G.N. Kuz'menko O.V., Laptev B.I., Gorlenko N.P., Antoshkin L.V. 2020. Ocenka mekhanizmov dejstviya i effektivnosti sochetannogo dejstviya foto i magnitoterapii (obzor literatury) [Evaluation of the Mechanisms of Action and Effectiveness of the Combined Action of Photo and Magnetic Therapy (Literature Review)]. Vestnik novyh medicinskih tekhnologij, 14 (6): 100–109. doi: 10.24411/2075-4094-2020-16730.
Terehov A.G., Grigorjan A.J., Mishina E.S. 2023. Sposob modelirovanija kozhnoj rany v uslovijah ishemii [Method for Modeling a Skin Wound under Ischemic Conditions]. Patent RF № 2807383. МПК A61D17/00 (2006.01), G09B 23/28 (2006.01). 32: 12.
Tush E.V., Eliseeva T.I., Haleckaja O.V., Krasil'nikova S.V., Ovsjannikov D.Ju., Potemina T.E., Ignatov S.K. 2019. Markery sostojanija jekstracelljuljarnogo matriksa i metody ih issledovanija (obzor) [Markers of the State of the Extracellular Matrix and Methods of their Study (Review)]. Sovremennye tehnologii v medicine, 11(2): 133–149. doi: 10.17691/stm2019.11.2.20.
Cissell D.D., Link J.M., Hu J.C., Athanasiou K.A.A. 2017. Modified Hydroxyproline Assay Based on Hydrochloric Acid in Ehrlich's Solution Accurately Measures Tissue Collagen Content. Tissue Eng. Part. C. Methods, 23(4): 243–250. doi: 10.1089/ten.tec.2017.0018.
Dini V., Granieri G., Michelucci A., Romanelli M. 2023. «Wound Measurement is an Essential Part of Wound Management». Chronic Wound Management: The Significance of Evidence and Technology. Cham: Springer International Publishing: 263–283. doi: 10.1007/978-3-031-26110-7_13.
Iljukhin E.A., Ivanov K.E., Micheev M.M. 2020. Lesion Meter: the Mobile App for the Ulcers Surface Area Measuring. URL: https://phlebounion.ru/en/projects#popup=/en/projects/mobilnoye-prilozheniye-izmereniya-ploshchadi-yazv-lesionmeter-5 (accessed: 17 February 2023).
Golaraei A., Mirsanaye K., Ro Y., Krouglov S., Akens M.K., Wilson B.C., Barzda V. 2019. Collagen Chirality and Three-Dimensional Orientation Studied with Polarimetric Second-Harmonic Generation Microscopy. J Biophotonics, 12(1). https://onlinelibrary.wiley.com/doi/full/10.1002/jbio.201800241 (accessed 17 February 2023). doi: 10.1002/jbio.201800241.
Moradi, M., Sabiza S., Rezaie A., Ezzati-Givi M. 2023. Investigating the Effect of Pentoxifylline and Zinc Oxide Combination on Experimental Full-Thickness Wound Healing in Rats. Iranian Journal of Veterinary Surgery, 18(1): 8–17. doi: 10.30500/ivsa.2022.355334.1313.
Najafi E., Ahmadi M., Mohammadi M., Beigmohammadi M.T., Heidary Z., Vatanara A., Khalili H. 2018. Topical Pentoxifylline for Pressure Ulcer Treatment: a Randomised, Double-Blind, Placebo-Controlled Clinical Trial. Journal of Wound Care, 27(8): 495–502. doi: 10.12968/jowc.2018.27.8.495.
O’Brien J., Parker C.N., Bui U., MacAndrew M., Mitchell J., Finlayson K.J. 2024. What is the Evidence on Skin Care for Maintaining Skin Integrity and Prevention of Wounds? An Integrative Review. Wound Practice & Research: Journal of the Australian Wound Management Association, 32(1): 25–33. doi: 10.33235/wpr.32.1.25-33.
Pasek J., Szajkowski S., Cieślar G. 2023. Application of Topical Hyperbaric Oxygen Therapy and Medical Active Dressings in the Treatment of Arterial Leg Ulcers. A Pilot Study. Sensors, 23(12): 5582. doi: 10.3390/s23125582.
Shukla S.K., Sharma A.K., Gupta V., Yashavarddhan M.H. 2019. Pharmacological Control of Inflammation in Wound Healing. Journal of Tissue Viability, 28(4): 218–222. doi: 10.1016/j.jtv.2019.09.002.
The work was carried out without external sources of funding.