The aim of the study was to analyze the level of hydroxysoplin in tissues as a marker of healing efficiency of experimental skin wound under ischemia conditions. A wound was reproduced according to our developed method on 90 rats Vistar. There were 3 groups of rats of 30 individuals. Therapy was not carried out in the group «Control», local treatment with multi-component means was performed in the «First experimental» group, magnetotherapy was performed with the above-mentioned agent in the «Second experimental» group. The hydroxylol content was estimated based on Newman’s, Logan’s technique modified by Cissel. The results were compared with planimetric, visual and morphological data. The formation of scar was observed in each series on the 10th day, there was recorded the predominance of mature connective tissue over granulation in the experimental groups in contrast to the «Control» group. There was a strong correlation between the hydroxyprolin content and the wound area in all groups. The article marked the reliability of the method of quantitative analysis of hydroxyproline, which made it possible to increase the objectivity of the representation of the course of the wound process in conjunction with other methods of research.
The aim of the study was to analyze the level of hydroxysoplin in tissues as a marker of healing efficiency of experimental skin wound under ischemia conditions. A wound was reproduced according to our developed method on 90 rats Vistar. There were 3 groups of rats of 30 individuals. Therapy was not carried out in the group «Control», local treatment with multi-component means was performed in the «First experimental» group, magnetotherapy was performed with the above-mentioned agent in the «Second experimental» group. The hydroxylol content was estimated based on Newman’s, Logan’s technique modified by Cissel. The results were compared with planimetric, visual and morphological data. The formation of scar was observed in each series on the 10th day, there was recorded the predominance of mature connective tissue over granulation in the experimental groups in contrast to the «Control» group. There was a strong correlation between the hydroxyprolin content and the wound area in all groups. The article marked the reliability of the method of quantitative analysis of hydroxyproline, which made it possible to increase the objectivity of the representation of the course of the wound process in conjunction with other methods of research.
The work was carried out without external sources of funding.